Lab resources

Methods

Misc

1. The NEW! codon wrangler
2. The amazing protein calculator
3. Column calibration & calculation
4. Common buffers
5. French Pressing
6. Western blotting - submersed method
7. Sel-met labelling of protein
8. NMR/minimal media solutions
9. Preparation of phenol/phenol:chloroform

Purification methods

1. p38_t
2. FxFG repeat
3. GST-Nup100
4. GST-Nup116
5. MexC2
6. Mtr2
7. p15
8. p47
9. TapC (incomplete)
10. TapC2 & Mutants
    • TapC2
    • TapC2 - Y47D
    • TapC2 - W45A

Useful stuff

1. Nucleic Acid Calculator
2. Codon usage table
3. Table of chemical shifts
4. Nups and mRNA export
5. NTF2/xxFG binding summary
6. Centriprep/centricon 'quickstart' guide
7. BCA protein determination template
8. -85 °C freezer contents

 

Useful places

• Protein parameters - includes pI and absorbance