The optimum, from a statistical analysis point of view, is that for any single-copy marker, 50% of the aliquots should be positive. This maximises the information that can be derived from the pattern matching.

The panel DNA is extracted and purified whilst embedded within LMP agarose strings by mixing the intact cells with molten agarose and allowing the mix to solidify within short glass capillaries. The extruded string can be manipulated and subjected to lysis, protein K digestion and buffer changes without significant damage to DNA integrity.

Estimates of content can be derived from the quantity of starting material and the dilutions used. This can give a rough guide to the genome equivalent quantity per unit length of string. A more accurate assessment, however, is needed and this is best done experimentally.

An initial test panel should be prepared and the GPA determined by typing two closely linked markers (in a multiplexed nested PCR reaction) and verifying similarity of pattern and calculating the GPA from the number of positive aliquots. The quantity of DNA used can be modified, if necessary, before making the master panel.