Mapping changes in copy number

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Using sensitive PCR techniques to detect single DNA molecules, we can accurately count how many copies of a given sequence are present in a sample. In this way, we can detect deletions, duplications or amplifications of parts of the genome.

These methods can be used not just to look for duplicated segments in ‘normal’ genomes, but to identify and locate the changes which occur in many types of cancer. This work is being developed in collaboration with the groups of Dr. Terry Rabbitts at LMB, and Dr. Pamela Rabbitts at UCL

Basic techniques for copy-number mapping