Supra-domains - evolutionary units larger than single protein domains

Christine Vogel¹ *,Carlo Berzuini², Matthew Bashton¹, Julian Gough³, Sarah A. Teichmann<sup>1

1</sup>MRC Laboratory of Molecular Biology, Cambridge, CB2 2QH, UK; ²MRC Biostatistics Unit, Cambridge, CB2 2QH, UK; ³Genome Exploration Research Group, RIKEN Genomic Sciences Centre, W121 1-7-22 Suehiro-cho, Tsurumi-ki, Yokohama 230-0045, Japan , and Department of Structural Biology, Fairchild bldg, D109, Stanford, CA 94305-5126, USA.

Manuscript.

Domains are the evolutionary units that proteins are composed of. Most proteins consist of several domains, and we examined to what extent domain combinations are conserved in different multi-domain proteins in the three kingdoms of life. We called domain combinations that occur with different partner domains supra-domains; and an example is shown here.

We characterised these supra-domains in 131 genomes with respect to

• their duplication (the number of proteins in which they occur)
• their recombination (versatility in terms of different domains at the N- and C-termini of the supra-domains)
• their overrepresentation with respect to abundance of the constituent domains.
• their functions and the spatial relationships of the constituent domains in supra-domains

Background information

Supplementary Figures and Tables

• Table S1. Occurrence of different sets of supra-domains in all sequences and domain architectures in the 131 genomes (coverage).
• Table S2. Functional and spatial relationships between domains of the principal supra-domains shared by bacteria, archaea and eukaryotes
• Table S3. Set overlaps of domains and supra-domains (with respect to the three kingdoms of life).
• Table S4. Versatility of domains and supra-domains (with respect to the number of different N- and C-terminal partner domains).
• List. Distribution across functional categories and classes .

Date and Results